Journal: PLoS ONE

Article Title: AdipoRon, adiponectin receptor agonist, improves vascular function in the mesenteric arteries of type 2 diabetic mice

doi: 10.1371/journal.pone.0230227

Figure Lengend Snippet: (A) CTR: control mice treated with vehicle, DB: diabetic mice treated with vehicle, and DB+ADI (AdipoRon): diabetic mice treated with AdipoRon. Representative western blot analysis and quantitative data for MYPT1 in mesenteric arteires (n = 4). (B) Vehicle: VSMCs stimulated with vehicle, PE: VSMCs stimulated with phenylephrine (5 μM), PE+ADI: VSMCs co-stimulated with PE (5 μM) and AdipoRon (ADI, 50 μM). Representative western blot analysis and quantitative data for MYPT1 in VSMCs (n = 4). Each group of blots was cropped from a same gel and membrane. * P < 0.05.

Article Snippet: Western blot analysis was performed for AdiR1 (1:1000 dilution; Abcam #ab70362, Cambridge, MA, USA), AdiR2 (1:1000 dilution; Abcam #ab77613, Cambridge, MA, USA), APPL1 (1:1000 dilution; Santa Cruz Biotechnology #SC-271901, Santa Cruz, CA, USA), APPL2 (1:1000 dilution; Santa Cruz Biotechnology #SC-271084, Santa Cruz, CA, USA), phosphorylated AMPK (1:1000 dilution; Abcam #2532s, Cambridge, MA, USA), total AMPK (1:1000 dilution; Abcam #2535s, Cambridge, MA, USA), phosphorylated eNOS (1:1000 dilution; Cell signaling #9571, Danvers, MA, USA), total eNOS (1:1000 dilution; Cell signaling #9586s, Danvers, MA, USA), and phosphorylated MYPT1 (myosin phosphatase target subunit 1, 1:500 dilution; Bioss #bs-3288R, Woburn, MA, USA), and total MYPT1 (1:500 dilution; Bioss #bs-3787R, Woburn, MA, USA).

Techniques: Western Blot